Emulsion PCR: a high efficient way of PCR amplification of random DNA libraries in aptamer selection.

Aptamers are short RNA or DNA oligonucleotides which can bind with different targets. Typically, they are selected from a large number of random DNA sequence libraries. The main strategy to obtain aptamers is systematic evolution of ligands by exponential enrichment (SELEX). Low efficiency is one of...

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Bibliographic Details

Published in:	PLoS ONE
Main Authors:	Keke Shao, Weifeng Ding, Feng Wang, Haiquan Li, Da Ma, Huimin Wang
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2011-01-01
Online Access:	http://europepmc.org/articles/PMC3174225?pdf=render