Comprehensive antibiotic-linked mutation assessment by resistance mutation sequencing (RM-seq)

Abstract Mutation acquisition is a major mechanism of bacterial antibiotic resistance that remains insufficiently characterised. Here we present RM-seq, a new amplicon-based deep sequencing workflow based on a molecular barcoding technique adapted from Low Error Amplicon sequencing (LEA-seq). RM-seq...

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Bibliographic Details

Published in:	Genome Medicine
Main Authors:	Romain Guérillot, Lucy Li, Sarah Baines, Brian Howden, Mark B. Schultz, Torsten Seemann, Ian Monk, Sacha J. Pidot, Wei Gao, Stefano Giulieri, Anders Gonçalves da Silva, Anthony D’Agata, Takehiro Tomita, Anton Y. Peleg, Timothy P. Stinear, Benjamin P. Howden
Format:	Article
Language:	English
Published:	BMC 2018-08-01
Subjects:	Antibiotic resistance Resistance mutations Deep sequencing Staphylococcus aureus Mycobacterium tuberculosis Rifampicin
Online Access:	http://link.springer.com/article/10.1186/s13073-018-0572-z