A marker-free genetic manipulation method for Glaesserella parasuis strains developed by alternately culturing transformants at 37°C and 30°C

Abstract Background Glaesserella parasuis (G. parasuis) is the causative agent of Glässer’s disease, which causes significant economic losses in the swine industry. However, research on the pathogenesis of G. parasuis has been hampered by the lack of a simple and efficient marker-free knockout syste...

وصف كامل

التفاصيل البيبلوغرافية
الحاوية / القاعدة:BMC Biotechnology
المؤلفون الرئيسيون: Jing Xiao, Yuxin Wang, Dongfang Wu, Yuping Song, Xuwang Cai, Huanchun Chen, Hongbo Zhou, Xiaojuan Xu
التنسيق: مقال
اللغة:الإنجليزية
منشور في: BMC 2024-09-01
الموضوعات:
G. Parasuis
Homologous recombination
Temperature-sensitive plasmids
Marker-free knockout
الوصول للمادة أونلاين:https://doi.org/10.1186/s12896-024-00887-w

الانترنت

https://doi.org/10.1186/s12896-024-00887-w

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