Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context

Summary: Recent technical advances, such as single-cell RNA sequencing and mass cytometry, improve identification of cell types and subsets in a range of healthy and diseased tissues at the expense of their cellular and molecular context. Here, we present a protocol for in situ multispectral imaging...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Elias B. Wieland, Laura J.A.P. Kempen, Chang Lu, Marjo M.P.C. Donners, Erik A.L. Biessen, Pieter Goossens
Format:	Article
Language:	English
Published:	Elsevier 2023-12-01
Subjects:	Single Cell Immunology Microscopy Molecular Biology Antibody
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723005683