PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA, which allows for the one-step assembly of multiple...

詳細記述

書誌詳細

出版年:	Cells
主要な著者:	Guoliang Yuan, Stanton Martin, Md Mahmudul Hassan, Gerald A. Tuskan, Xiaohan Yang
フォーマット:	論文
言語:	英語
出版事項:	MDPI AG 2022-08-01
主題:	gRNA array multiplexed CRISPR genome editing assembly method Golden Gate assembly PARA
オンライン･アクセス:	https://www.mdpi.com/2073-4409/11/16/2467