Improved constructs for bait RNA display in a bacterial three-hybrid assay

Abstract We have previously developed a transcription-based bacterial three-hybrid (B3H) assay as a genetic approach to probe RNA–protein interactions inside of E. coli cells. This system offers a straightforward path to identify and assess the consequences of mutations in RBPs with molecular phenot...

Full description

Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Linh D. Nguyen, Hannah LeBlanc, Katherine E. Berry
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-01-01
Subjects:	RNA–protein interactions Hfq Bacterial sRNAs B3H assay GC clamps
Online Access:	https://doi.org/10.1038/s41598-024-85082-9