Genome-wide distribution of RNA-DNA hybrids identifies RNase H targets in tRNA genes, retrotransposons and mitochondria.
During transcription, the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout the budding yeast genome. In wild-type strains, R-loops were readily detected...
| 發表在: | PLoS Genetics |
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| Main Authors: | , , , |
| 格式: | Article |
| 語言: | 英语 |
| 出版: |
Public Library of Science (PLoS)
2014-10-01
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| 在線閱讀: | https://doi.org/10.1371/journal.pgen.1004716 |