Robust single-nucleus RNA sequencing reveals depot-specific cell population dynamics in adipose tissue remodeling during obesity

Single-nucleus RNA sequencing (snRNA-seq), an alternative to single-cell RNA sequencing (scRNA-seq), encounters technical challenges in obtaining high-quality nuclei and RNA, persistently hindering its applications. Here, we present a robust technique for isolating nuclei across various tissue types...

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Bibliographic Details

Published in:	eLife
Main Authors:	Jisun So, Olivia Strobel, Jamie Wann, Kyungchan Kim, Avishek Paul, Dominic J Acri, Luke C Dabin, Jungsu Kim, Gang Peng, Hyun Cheol Roh
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2025-01-01
Subjects:	snRNA-seq adipocytes obesity
Online Access:	https://elifesciences.org/articles/97981