Correlative multiphoton-STED microscopy of podocyte calcium levels and slit diaphragm ultrastructure in the renal glomerulus

Abstract In recent years functional multiphoton (MP) imaging of vital mouse tissues and stimulation emission depletion (STED) imaging of optically cleared tissues allowed new insights into kidney biology. Here, we present a novel workflow where MP imaging of calcium signals can be combined with supe...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Eva Wiesner, Julia Binz-Lotter, Agnes Hackl, David Unnersjö-Jess, Nelli Rutkowski, Thomas Benzing, Matthias J. Hackl
Format:	Article
Language:	English
Published:	Nature Portfolio 2024-06-01
Online Access:	https://doi.org/10.1038/s41598-024-63507-9