High-Yield Expression and Purification of Scygonadin, an Antimicrobial Peptide, Using the Small Metal-Binding Protein SmbP

(1) Background: Producing active antimicrobial peptides with disulfide bonds in bacterial strains is challenging. The cytoplasm of <i>Escherichia coli</i> has a reducing environment, which is not favorable to the formation of disulfide bonds. Additionally, <i>E. coli</i> may...

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Bibliographic Details

Published in:	Microorganisms
Main Authors:	Jessica J. Gomez-Lugo, Nestor G. Casillas-Vega, Alma Gomez-Loredo, Isaias Balderas-Renteria, Xristo Zarate
Format:	Article
Language:	English
Published:	MDPI AG 2024-01-01
Subjects:	scygonadin SmbP antimicrobial peptides <i>Escherichia coli</i> <i>Staphylococcus aureus</i> immobilized metal-affinity chromatography
Online Access:	https://www.mdpi.com/2076-2607/12/2/278