Improving an rRNA depletion protocol with statistical design of experiments

In prokaryotic RNA-seq library preparation, rRNA depletion is required to remove highly abundant rRNA transcripts from total RNA. rRNA is so abundant that small improvements in depletion efficiency lead to large increases in mRNA sequencing coverage. The current gold-standard method for rRNA depleti...

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Bibliographic Details

Published in:	SLAS Technology
Main Authors:	Benjamin M. David, Paul A. Jensen
Format:	Article
Language:	English
Published:	Elsevier 2023-02-01
Subjects:	RNA-seq Design of experiments Process improvement NGS library preparation
Online Access:	http://www.sciencedirect.com/science/article/pii/S2472630322051767