Protocol for label-free ribosome-associated protein enrichment from mammalian cells by RAPIDASH

Summary: Ribosome-associated proteins (RAPs) enable modulation of gene expression at the level of mRNA translation in response to cellular needs. Here, we describe a method called ribosome-associated protein identification by affinity to sulfhydryl-charged resin (RAPIDASH) for tag-free isolation of...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Victoria Hung, Teodorus Theo Susanto, Maria Barna
Format:	Article
Language:	English
Published:	Elsevier 2025-09-01
Subjects:	Cell Biology Molecular Biology Protein Biochemistry
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725004587