Highly efficient construction of infectious viroid-derived clones
Abstract Background Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that presents several disadvantages: (i) low efficiency, (ii) it is a non-oriente...
| Published in: | Plant Methods |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-08-01
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| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13007-019-0470-4 |