A novel MoClo-mediated intron insertion system facilitates enhanced transgene expression in Chlamydomonas reinhardtii

The Chlamydomonas Modular Cloning (MoClo) toolkit allows for straightforward and flexible construction of genetic modules for gene expression in the microalgal model species, fostering developments in algal biotechnology. Efficiently expressing transgenes from the nuclear genome of C. reinhardtii re...

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Bibliographic Details

Published in:	Frontiers in Plant Science
Main Authors:	Moritz Aschern, Jochem Braad, Alfonsina Milito, David Alzuria, Jae-Seong Yang
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-03-01
Subjects:	synthetic biology Chlamydomonas reinhardtii transgene expression intron-mediated enhancement (IME) Modular Cloning (MoClo) microalgal biotechnology
Online Access:	https://www.frontiersin.org/articles/10.3389/fpls.2025.1544873/full