Enhanced Production of ECM Proteins for Pharmaceutical Applications Using Mammalian Cells and Sodium Heparin Supplementation

The yields of soluble ECM proteins recombinantly produced with mammalian cells can be significantly enhanced by exploiting the stabilizing properties of heparin. Here, we propose a simple and straightforward scalable protocol for the mammalian cell production of ECM proteins with affinity for hepari...

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Bibliographic Details

Published in:	Pharmaceutics
Main Authors:	Javier Garcia-Pardo, Sergi Montané, Francesc Xavier Avilés, Sebastian Tanco, Julia Lorenzo
Format:	Article
Language:	English
Published:	MDPI AG 2022-10-01
Subjects:	ECM proteins sodium heparin heparin binding enhanced protein expression carboxypeptidases mammalian cells
Online Access:	https://www.mdpi.com/1999-4923/14/10/2138