Spectral phasor imaging on a commercial confocal microscope without a spectral detector

Abstract Spectral imaging is a fluorescence microscopy technique with several applications, including imaging of environment-sensitive probes, spectral unmixing and identification of fluorescent species. In confocal microscopes not equipped with a spectral detection unit, spectral images can be obta...

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Bibliographic Details

Published in:	Scientific Reports
Main Authors:	Elisa Longo, Angelita Costantino, Alessio Andreoni, Paolo Bosco, Anna P. Privitera, Silvia Scalisi, Leonel Malacrida, Massimo Gulisano, Luca Lanzanò
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-08-01
Subjects:	Spectral phasor Confocal microscope Environment-sensitive probes ACDAN Nile red Fluorescent proteins
Online Access:	https://doi.org/10.1038/s41598-025-15637-x