Study on the effect of nine nucleic acid extraction lysing reagents on PCR amplification efficiency

ObjectiveTo explore the maximum allowable concentration of six chemical reagents and three enzyme lysates in a polymerase chain reaction （PCR） amplification system and the differences of IGEPAL CA-630 cleaving different species.MethodsPrepare different kinds of working solutions， including six chemi...

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Bibliographic Details

Published in:	Zhongguo shipin weisheng zazhi
Main Authors:	LIU Na, WANG Yaping, WANG Xueshuo, CUI Shenghui
Format:	Article
Language:	Chinese
Published:	The Editorial Office of Chinese Journal of Food Hygiene 2023-06-01
Subjects:	polymerase chain reaction lysing reagent chemical trial dosage form enzymatic species
Online Access:	http://www.zgspws.com/zgspwszz/article/abstract/202306007