HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi

This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi. pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals...

Full description

Bibliographic Details
Published in:Memorias do Instituto Oswaldo Cruz
Main Authors: Otávio T Nóbrega, Jaime M Santana, Nancy R Sturm, Antônio RL Teixeira
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2004-08-01
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000500010