Detecting in-solution conformational changes in viral fusogens using tryptophan-induced fluorescence quenching

Summary: Dynamic monitoring of protein conformational changes is necessary to fully understand many biological processes. For example, viral entry and membrane fusion require rearrangement of its viral glycoprotein. We present a step-by-step protocol for site-specific bimane labeling of the influenz...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Vitor Hugo B. Serrão, Jeffrey E. Lee
Format:	Article
Language:	English
Published:	Elsevier 2021-12-01
Subjects:	Biophysics Microbiology Molecular/Chemical Probes Protein Biochemistry Protein expression and purification Structural Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721007000