Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing

Research background. Cascara, the dried husk of coffee cherries, has attracted attention as a potential beverage due to its unique flavour profile and potential health benefits. Traditionally, cascara is prepared using hot brewing methods. However, recent interest in cold brewing methods has led to...

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出版年:Food Technology and Biotechnology
主要な著者: Sali Muriqi, Libor Červenka, Lenka Česlová, Michal Kašpar, Soňa Řezková, Lenka Husáková, Jan Patočka, Petr Česla, Helena Velichová
フォーマット: 論文
言語:英語
出版事項: University of Zagreb Faculty of Food Technology and Biotechnology 2025-01-01
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オンライン・アクセス:https://hrcak.srce.hr/file/477115
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author Sali Muriqi
Libor Červenka
Lenka Česlová
Michal Kašpar
Soňa Řezková
Lenka Husáková
Jan Patočka
Petr Česla
Helena Velichová
author_facet Sali Muriqi
Libor Červenka
Lenka Česlová
Michal Kašpar
Soňa Řezková
Lenka Husáková
Jan Patočka
Petr Česla
Helena Velichová
author_sort Sali Muriqi
collection DOAJ
container_title Food Technology and Biotechnology
description Research background. Cascara, the dried husk of coffee cherries, has attracted attention as a potential beverage due to its unique flavour profile and potential health benefits. Traditionally, cascara is prepared using hot brewing methods. However, recent interest in cold brewing methods has led to research on how temperature affects the functional properties of cascara beverages. Experimental approach. Colour (CIE L*a*b*), total dissolved solids and titratable acidity were determined in cascara beverages prepared at 5, 10, 15 and 20 °C. The concentration of phenols and flavonoids, as well as antioxidant properties were evaluated using spectrophotometric methods. Caffeine, chlorogenic acid and melanoidins were quantified by HPLC. The mineral composition was determined using inductively coupled plasma mass spectrometry (ICP-MS). The results were compared with a hot-brewed cascara beverage. Results and conclusions. Cold brewing resulted in significantly higher concentrations of total phenolic compounds, expressed as gallic acid equivalents (ranging from 309 to 354 mg/L), total flavonoids, expressed as quercetin equivalents (11.8–13.6 mg/L), and caffeine (123–136 mg/L) than the hot-brewed cascara beverage sample (p<0.05). Temperature had a noticeable effect on most variables, although the effect appeared to be random. In particular, concentrations of caffeine (p<0.01) and copper (p<0.001) were highest in beverages prepared at 20 °C and decreased with decreasing brewing temperature. Multivariate analysis showed that minerals (As, Co, Mn, Sn, Mg and Ca), hue and phenolic concentration contributed to the first principal component, which mainly differentiated the hot-brewed sample. Antioxidant-related variables, total titratable acidity and Se contributed most to the second principal component, which facilitated the separation of samples brewed at 5 °C. Novelty and scientific contribution. To our knowledge, this is the first study to suggest that temperature affects the functional properties of cascara beverage produced by the cold brewing method. Experimental evidence supports the existence of a direct proportionality between caffeine and copper concentrations and brewing temperature.
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spelling doaj-art-8c8a9cb287d84a7ebd4aa1fc8e9c29952025-08-20T02:19:50ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062025-01-01631465610.17113/ftb.63.01.25.8605Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold BrewingSali Muriqi0Libor Červenka1Lenka Česlová2Michal Kašpar3Soňa Řezková4Lenka Husáková5Jan Patočka6Petr Česla7Helena Velichová8Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, Pardubice 53210, Czech RepublicDepartment of Food Analysis and Chemistry, Faculty of Technology, Tomáš Bata University in Zlín, nám. T. G. Masaryka 5555, 460 01 Zlín, Czech RepublicResearch background. Cascara, the dried husk of coffee cherries, has attracted attention as a potential beverage due to its unique flavour profile and potential health benefits. Traditionally, cascara is prepared using hot brewing methods. However, recent interest in cold brewing methods has led to research on how temperature affects the functional properties of cascara beverages. Experimental approach. Colour (CIE L*a*b*), total dissolved solids and titratable acidity were determined in cascara beverages prepared at 5, 10, 15 and 20 °C. The concentration of phenols and flavonoids, as well as antioxidant properties were evaluated using spectrophotometric methods. Caffeine, chlorogenic acid and melanoidins were quantified by HPLC. The mineral composition was determined using inductively coupled plasma mass spectrometry (ICP-MS). The results were compared with a hot-brewed cascara beverage. Results and conclusions. Cold brewing resulted in significantly higher concentrations of total phenolic compounds, expressed as gallic acid equivalents (ranging from 309 to 354 mg/L), total flavonoids, expressed as quercetin equivalents (11.8–13.6 mg/L), and caffeine (123–136 mg/L) than the hot-brewed cascara beverage sample (p<0.05). Temperature had a noticeable effect on most variables, although the effect appeared to be random. In particular, concentrations of caffeine (p<0.01) and copper (p<0.001) were highest in beverages prepared at 20 °C and decreased with decreasing brewing temperature. Multivariate analysis showed that minerals (As, Co, Mn, Sn, Mg and Ca), hue and phenolic concentration contributed to the first principal component, which mainly differentiated the hot-brewed sample. Antioxidant-related variables, total titratable acidity and Se contributed most to the second principal component, which facilitated the separation of samples brewed at 5 °C. Novelty and scientific contribution. To our knowledge, this is the first study to suggest that temperature affects the functional properties of cascara beverage produced by the cold brewing method. Experimental evidence supports the existence of a direct proportionality between caffeine and copper concentrations and brewing temperature.https://hrcak.srce.hr/file/477115cascaracold brewingtemperature effectcaffeine
spellingShingle Sali Muriqi
Libor Červenka
Lenka Česlová
Michal Kašpar
Soňa Řezková
Lenka Husáková
Jan Patočka
Petr Česla
Helena Velichová
Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing
cascara
cold brewing
temperature effect
caffeine
title Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing
title_full Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing
title_fullStr Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing
title_full_unstemmed Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing
title_short Physicochemical, Antioxidant and Mineral Composition of Cascara Beverage Prepared by Cold Brewing
title_sort physicochemical antioxidant and mineral composition of cascara beverage prepared by cold brewing
topic cascara
cold brewing
temperature effect
caffeine
url https://hrcak.srce.hr/file/477115
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