CRISPR-SKIP: programmable gene splicing with single base editors

Abstract CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mu...

Full description

Bibliographic Details

Published in:	Genome Biology
Main Authors:	Michael Gapinske, Alan Luu, Jackson Winter, Wendy S. Woods, Kurt A. Kostan, Nikhil Shiva, Jun S. Song, Pablo Perez-Pinera
Format:	Article
Language:	English
Published:	BMC 2018-08-01
Subjects:	Gene editing Base editing Exon skipping Alternative splicing Synthetic biology CRISPR-Cas9
Online Access:	http://link.springer.com/article/10.1186/s13059-018-1482-5