Efficient Expression and Enzymatic Properties of L-Threonine Dehydrogenase from Escherichia coli

Our aim was to improve the catalytic efficiency of L-threonine dehydrogenase (L-TDH) on L-threonine dehydrogenation to synthesize ethyl L-2-aminoacetate. An L-TDH gene from Escherichia coli was mined and solubly expressed in E. coli BL21(DE3) through pACYCDuet-1 expression system. The expressed enzy...

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Bibliographic Details

Published in:	Shipin Kexue
Main Author:	LIU Xinxin, WANG Yao, SHI Hongling, YAO Lunguang, WANG Xian, TANG Cunduo
Format:	Article
Language:	English
Published:	China Food Publishing Company 2023-09-01
Subjects:	l-threonine; l-threonine dehydrogenase; escherichia coli; expression; biotransformation
Online Access:	https://www.spkx.net.cn/fileup/1002-6630/PDF/2023-44-18-011.pdf