An Inducible Lentiviral Guide RNA Platform Enables the Identification of Tumor-Essential Genes and Tumor-Promoting Mutations In Vivo

The CRISPR/Cas9 technology enables the introduction of genomic alterations into almost any organism; however, systems for efficient and inducible gene modification have been lacking, especially for deletion of essential genes. Here, we describe a drug-inducible small guide RNA (sgRNA) vector system...

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Bibliographic Details

Published in:	Cell Reports
Main Authors:	Brandon J. Aubrey, Gemma L. Kelly, Andrew J. Kueh, Margs S. Brennan, Liam O’Connor, Liz Milla, Stephen Wilcox, Lin Tai, Andreas Strasser, Marco J. Herold
Format:	Article
Language:	English
Published:	Elsevier 2015-03-01
Online Access:	http://www.sciencedirect.com/science/article/pii/S2211124715001102