Protocol for reproducible EZ clearing and labeling, including optimized steps and quantified fluorescence retention on mouse tissue

Summary: Here, we present a protocol for preserving fluorescent markers using tissue clearing and immunolabeling in mouse tissue such as sciatic nerve, gut, and spinal cord. We describe steps for maintaining tetrahydrofuran (THF) under an inert atmosphere, EZ clearing, immunolabeling, imaging with c...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Ivana Gantar, Jade Nguyen, Dimokratis Karamanlis, Steven Ceto, Samira F. Osterop, Domitille Rajot, Sami El-Boustani, Laura Batti
Format:	Article
Language:	English
Published:	Elsevier 2025-12-01
Subjects:	Cell Biology Microscopy Neuroscience Tissue Engineering
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725005118