A novel KRAS exon 2 drop-off digital PCR assay for mutation detection in cell-free DNA of cancer patients

Abstract Background KRAS exon 2 mutations are highly prevalent in human malignancies, making them attractive targets for detection and monitoring in cell-free DNA (cfDNA) of cancer patients. Drop-off assays designed for digital polymerase chain reaction (ddPCR drop-off) span entire mutational hotspo...

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Bibliographic Details

Published in:	Diagnostic Pathology
Main Authors:	Bianca Addamo-De Nard, Meret Geissmann, Dilara Akhoundova, Clelia Pistoni, Tomas Brezina, Martin Zoche, Achim Weber, Saskia Hussung, Ralph Fritsch
Format:	Article
Language:	English
Published:	BMC 2025-05-01
Subjects:	Liquid biopsy Droplet digital polymerase chain reaction (ddPCR) KRAS Drop-off assay Cell-free DNA (cfDNA) Circulating-tumor DNA (ctDNA)
Online Access:	https://doi.org/10.1186/s13000-025-01637-y