A high efficiency precision genome editing method with CRISPR in iPSCs

Abstract The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small molecules achieves a homologous reco...

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التفاصيل البيبلوغرافية

الحاوية / القاعدة:	Scientific Reports
المؤلفون الرئيسيون:	Avinash Singh, G. Dalton Smedley, Jamee-Grace Rose, Kristina Fredriksen, Ying Zhang, Ling Li, Shauna H. Yuan
التنسيق:	مقال
اللغة:	الإنجليزية
منشور في:	Nature Portfolio 2024-04-01
الموضوعات:	CRISPR iPSC Gene editing High efficiency Single nucleotide polymorphism
الوصول للمادة أونلاين:	https://doi.org/10.1038/s41598-024-60766-4