Quantitative bead–based multiplex assay for simultaneous determination of IgG concentrations of pertussis toxin, filamentous hemagglutinin, pertactin, diphtheria, tetanus, Haemophilus influenzae b, and hepatitis B in human serum samples

BackgroundMultiplex serological assays provide opportunities for seroprevalence studies and for evaluating antibodies post-vaccination. In this report, we describe the development and validation of a seven-plex bead-based assay for quantifying human immunoglobulin G (IgG) antibodies against pertussi...

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Bibliographic Details

Published in:	Frontiers in Immunology
Main Authors:	Vishal Rathod, Sagar Katke, Sumant Patil, Sachin Bhandare, Laxmikant Kadam, Manish Gautam, Prabhu Gumma, Krishna Manoj Kumar, Laura Hassall, Cathy Asokanathan, Alex Douglas-Bardsley, Kevin Markey, Sumit Gupta, Harish Rao, Sameer Parekh, Pramod Pujari, Hitt Sharma, Umesh Shaligram, Sunil Gairola
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-07-01
Subjects:	ELISA luminex multiplex immunoassay combination vaccines Haemophilus influenzae B Pertussis
Online Access:	https://www.frontiersin.org/articles/10.3389/fimmu.2025.1587567/full