Active sites and thermostability of a non-specific nuclease from Yersinia enterocolitica subsp. palearctica by site-directed mutagenesis

To identify the amino acid residues that are the critical factors contributing to the high catalytic activity and good thermostability of Yersinia enterocolitica subsp. palearctica non-specific nuclease (Y. NSN), in this work, we analyzed the structure and the functional domain of Y. NSN by using bi...

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Bibliographic Details

Published in:	Biotechnology & Biotechnological Equipment
Main Authors:	Yu Zhang, Tao Chen, Wei Zheng, Zhen Hua Li, Rui-Feng Ying, Zhen-Xing Tang, Lu-E Shi
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2018-09-01
Subjects:	Enzyme activity DNA dynamic modelling viability Yersinia enterocolitica subsp. palearctica site-directed mutagenesis
Online Access:	http://dx.doi.org/10.1080/13102818.2018.1489738