Glyoxal-based fixation of Drosophila embryos for immunofluorescence staining and RNA in situ hybridization

Summary: The dialdehyde glyoxal is an alternative chemical fixative that cross-links tissues faster than formaldehyde, retains higher antigenicity, and is less hazardous than either formaldehyde or glutaraldehyde. Here we present a glyoxal-based fixation protocol for use with Drosophila embryos. We...

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書誌詳細

出版年:	STAR Protocols
主要な著者:	Shrunali Amin, Malika Basu, Valeria Buzinova, Anthony Delgado, Tejas Mahadevan, Sanya Mishra, Sarah Zaida, Xi Wang, Anna Marie Sokac
フォーマット:	論文
言語:	英語
出版事項:	Elsevier 2023-09-01
主題:	Cell Biology Developmental Biology Microscopy
オンライン･アクセス:	http://www.sciencedirect.com/science/article/pii/S2666166723003520