Screening self-peptides for recognition by mouse alloreactive CD8+ T cells using direct ex vivo multimer staining

Summary: Here, we present a protocol to identify immunogenic self-peptide/allogeneic major histocompatibility complex (MHC) epitopes. We describe the generation of enriched alloreactive CD8+ T cells by priming mice with a skin graft expressing the allogeneic MHC class I molecule of interest, followe...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Eric T. Son, Moumita Paul-Heng, Mario Leong, Chuanmin Wang, Alexandra E. Hill, Martina Denkova, Anthony W. Purcell, Nicole A. Mifsud, Alexandra F. Sharland
Format:	Article
Language:	English
Published:	Elsevier 2023-03-01
Subjects:	Cell Biology Cell isolation Immunology Model Organisms Molecular Biology Antibody
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722008231