In Vivo Quantitative Estimation of DNA-Dependent Interaction of Sox2 and Oct4 Using BirA-Catalyzed Site-Specific Biotinylation

Protein−protein interactions of core pluripotency transcription factors play an important role during cell reprogramming. Cell identity is controlled by a trio of transcription factors: Sox2, Oct4, and Nanog. Thus, methods that help to quantify protein−protein interactions may be...

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Bibliographic Details

Published in:	Biomolecules
Main Authors:	Arman Kulyyassov, Vasily Ogryzko
Format:	Article
Language:	English
Published:	MDPI AG 2020-01-01
Subjects:	protein–protein interactions (ppi) proximity-utilizing biotinylation (pub) biotin acceptor peptide (bap) bira proteomics liquid chromatography–tandem mass spectrometry (lc–ms/ms) multiple reaction monitoring (mrm) in vivo dna-dependent protein–protein interaction pluripotency transcription factors sox2 and oct4
Online Access:	https://www.mdpi.com/2218-273X/10/1/142