Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry

RNA synthesis can be detected by 5-ethynyl uridine (EU) incorporation and click chemistry. Despite identifying a fundamental functional process, this technique has yet to be widely applied to complex human tissue systems. By incorporating EU into human hair follicle (HF) organs cultured ex vivo, nas...

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Bibliographic Details

Published in:	BioTechniques
Main Authors:	Talveen S Purba, Jack Marsh, Kayumba Ng'andu, Svitlana Kurinna, Ralf Paus
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2018-08-01
Subjects:	5-ethynyl uridine click chemistry human hair follicle O-propargyl-puromycin protein synthesis RNA synthesis
Online Access:	https://www.future-science.com/doi/10.2144/btn-2018-0025