An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

The plasmid vector pGreenII is widely used to produce plant transformants via a process that involves propagation in Escherichia coli. However, we show here that pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. In additio...

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發表在:	G3: Genes, Genomes, Genetics
Main Authors:	Michael R. Watson, Yu-fei Lin, Elizabeth Hollwey, Rachel E. Dodds, Peter Meyer, Kenneth J. McDowall
格式:	Article
語言:	英语
出版:	Oxford University Press 2016-07-01
主題:	Agrobacterium plant transformation pGreen E. coli growth defects
在線閱讀:	http://g3journal.org/lookup/doi/10.1534/g3.116.029405