Establishment of TUBB3-mCherry knock-in human pluripotent stem cell line using CRISPR/Cas9 (SNUe003-A-4)

TUBB3 is a structural neuronal protein important for multiple neuronal functions including axonal guidance and maturation. This study aimed to generate a human pluripotent stem cell (hPSC) line with a TUBB3-mCherry reporter using CRISPR/SpCas9 nuclease. The stop codon in the last exon of TUBB3 was r...

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Bibliographic Details

Published in:	Stem Cell Research
Main Authors:	A-Hyeon Kim, Ha Myoung Lee, Hong-Sik Kim, Juwon Jung, Hyewon Seol, Eunmi Choi, Seongeun Lee, Young Min Choi, Jong Kwan Jun, Han-Soo Kim, Jiho Jang
Format:	Article
Language:	English
Published:	Elsevier 2023-06-01
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506123000508