Improved Native Isolation of Endogenous Protein A-Tagged Protein Complexes

Here we report a modified peptide reagent useful for the rapid, native elution of protein complexes containing a Protein-A-tagged component. We tested this reagent for the elution of tagged endogenous protein complexes from yeast (Nup53p/Nup170p dimer; Nup1p/Kap95p/Kap60p trimer; pentameric GINS com...

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Bibliographic Details
Published in:BioTechniques
Main Authors: John LaCava, Nagarajan Chandramouli, Hua Jiang, Michael P. Rout
Format: Article
Language:English
Published: Taylor & Francis Group 2013-04-01
Subjects:
native elution
competitive elution
Protein A
SpA
PrA
affinity isolation
Online Access:https://www.future-science.com/doi/10.2144/000114012

Internet

https://www.future-science.com/doi/10.2144/000114012

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