Reverse transcription recombinase polymerase amplification-lateral flow assay for detection of pathogenic orthoflaviviruses in mosquito vectors

Background The genus Orthoflavivirus primarily consists of arthropod-borne viruses capable of infecting vertebrate hosts and causing serious human diseases such as dengue fever, Zika fever, Japanese encephalitis, West Nile fever, and yellow fever. This study describes the development of a simple and...

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Bibliographic Details

Published in:	PeerJ
Main Authors:	Parinda Thayanukul, Ronald Enrique Morales Vargas, Konkamon Sujijun, Pimchanok Khumpeera, Kittiya Suksawat, Nahallage Dona Asha Dilrukshi Wijegunawardana, Patsamon Rijiravanich, Werasak Surareungchai, Pattamaporn Kittayapong
Format:	Article
Language:	English
Published:	PeerJ Inc. 2025-08-01
Subjects:	Orthoflavivirus Reverse Transcription Recombinase Polymerase Amplification (RT-RPA) Lateral Flow Detection (LFD) Mosquito Arbovirus Recombinase Polymerase Amplification (RPA)
Online Access:	https://peerj.com/articles/19820.pdf