Glycoengineering of plant-produced Pembrolizumab enhances FcRn binding and extends serum half-life in mice

Plant systems offer scalable and cost-effective platforms for antibody production, but plant-specific glycans may affect pharmacokinetics and immunogenicity. To evaluate the impact of Fc glycosylation, four Pembrolizumab glycovariants were generated in Nicotiana benthamiana: wild-type glycosylation...

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Bibliographic Details

Published in:	Biotechnology Reports
Main Authors:	Christine Joy I. Bulaon, Janejira Jaratsittisin, Kaewta Rattanapisit, Pipob Suwanchaikasem, Shiying Guo, Khwanchit Boonha, Pannamthip Pitaksajjakul, Nipaporn Simsom, Vudhiporn Limprasutr, Waranyoo Phoolcharoen
Format:	Article
Language:	English
Published:	Elsevier 2025-12-01
Subjects:	Pembrolizumab Nicotiana benthamiana Glycoengineering Fc glycosylation FcRn binding Pharmacokinetics
Online Access:	http://www.sciencedirect.com/science/article/pii/S2215017X25000542