Engineering psychrophilic polymerase for nanopore long-read sequencing

Unveiling the potential application of psychrophilic polymerases as candidates for polymerase-nanopore long-read sequencing presents a departure from conventional choices such as thermophilic Bacillus stearothermophilus (Bst) renowned for its limitation in temperature and mesophilic Bacillus subtili...

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Bibliographic Details

Published in:	Frontiers in Bioengineering and Biotechnology
Main Authors:	Yaping Sun, Danny Hsu Ko, Jie Gao, Kang Fu, Yuanchen Mao, Yun He, Hui Tian
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2024-07-01
Subjects:	psychrophilic polymerases incorporation of polymer-tagged nucleotides salt tolerance exonuclease activity sequencing by synthesis (SBS)
Online Access:	https://www.frontiersin.org/articles/10.3389/fbioe.2024.1406722/full