Structural basis for target DNA cleavage and guide RNA processing by CRISPR-Casλ2

Abstract RNA-guided CRISPR-Cas nucleases are widely used as versatile genome-engineering tools. Among the diverse CRISPR-Cas effectors, CRISPR-Casλ—also referred to as Cas12n—is a recently identified miniature type V nuclease encoded in phage genomes. Given its demonstrated nuclease activity in both...

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Bibliographic Details

Published in:	Communications Biology
Main Authors:	Satoshi N. Omura, Lauren E. Alfonse, Alexa Ornstein, Hayato Morinaga, Hisato Hirano, Yuzuru Itoh, Gabrielle Munoz, Anthony J. Garrity, Gregory R. Hoffman, Tia DiTommaso, Winston X. Yan, David R. Cheng, David A. Scott, Zachary Maben, Osamu Nureki
Format:	Article
Language:	English
Published:	Nature Portfolio 2025-06-01
Online Access:	https://doi.org/10.1038/s42003-025-08300-8