Optimization of highly efficient exogenous-DNA-free Cas9-ribonucleoprotein mediated gene editing in disease susceptibility loci in wheat (Triticum aestivum L.)

The advancement of precision engineering for crop trait improvement is important in the face of rapid population growth, climate change, and disease. To this end, targeted double-stranded break technology using RNA-guided Cas9 has been adopted widely for genome editing in plants. Agrobacterium or pa...

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Bibliographic Details

Published in:	Frontiers in Plant Science
Main Authors:	Snigdha Poddar, Jaclyn Tanaka, Katherine L. D. Running, Gayan K. Kariyawasam, Justin D. Faris, Timothy L. Friesen, Myeong-Je Cho, Jamie H. D. Cate, Brian Staskawicz
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2023-01-01
Subjects:	wheat gene editing (CRISPR-Cas9) ribonucleoprotein (RNP) DNA-free transgene-free temperature treatment
Online Access:	https://www.frontiersin.org/articles/10.3389/fpls.2022.1084700/full