Evaluation of long-read 16S rRNA next-generation sequencing for identification of bacterial isolates in a clinical diagnostic laboratory

ABSTRACT Sanger sequencing of the first ~500 bp of the 16S rRNA gene is frequently used to identify bacterial pathogens that have ambiguous biochemical profiles or proteomic mass spectra. When diversity does not occur within that region, genus-level and/or species-level identification may not be pos...

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Bibliographic Details

Published in:	Journal of Clinical Microbiology
Main Authors:	Victoria L. Campodónico, Jean Ruelle, Anna Fitzgerald, Yehudit Bergman, Brenda Osborne, Dimitrios Bourdas, Jennifer Lu, Karen C. Carroll, Patricia J. Simner
Format:	Article
Language:	English
Published:	American Society for Microbiology 2025-05-01
Subjects:	16S rRNA Nanopore Sanger sequencing identification
Online Access:	https://journals.asm.org/doi/10.1128/jcm.01670-24