Ladder-shape melting temperature isothermal amplification of nucleic acids

A novel method, termed ladder-shape melting temperature isothermal amplification (LMTIA), was developed in this study. As a proof of concept, one pair of primers or two pairs of nested primers and a thermostable DNA polymerase were employed to amplify the internal transcribed spacer of Oryza sativa...

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Bibliographic Details
Published in:BioTechniques
Main Authors: Deguo Wang, Yongzhen Wang, Meng Zhang, Yongqing Zhang, Juntao Sun, Chunmei Song, Fugang Xiao, Yuan Ping, Chen Pan, Yushan Hu, Chaoqun Wang, Yanhong Liu
Format: Article
Language:English
Published: Taylor & Francis Group 2021-07-01
Subjects:
isothermal amplification
ladder-shape melting temperature isothermal amplification (LMTIA)
loop-mediated isothermal amplification (LAMP)
melting curve
nucleic acids detection
primer design
Online Access:https://www.future-science.com/doi/10.2144/btn-2020-0173

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https://www.future-science.com/doi/10.2144/btn-2020-0173

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