Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach

Summary: Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental des...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Nadia Krarup Knudsen, Christine Hjorth Andreassen, Martin Blomberg Jensen, Anne Jørgensen
Format:	Article
Language:	English
Published:	Elsevier 2025-06-01
Subjects:	Cell biology Cell culture Cell isolation Model organisms
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166725001376