Embedding a feruloyl esterase active site into a thermophilic endoxylanase scaffold for the degradation of feruloylated xylans

The structural complexity of xylan makes its complete degradation challenging. Strategies to improve its hydrolysis often requires enzyme cocktails with multiple specific activities or proteins harboring multiple catalytic domains. Here, we introduce a novel approach through the design of Xyn11m1, a...

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Bibliographic Details

Published in:	Computational and Structural Biotechnology Journal
Main Authors:	Rubén Muñoz-Tafalla, Isabel Cea-Rama, Fadia V. Cervantes, Jose L. Gonzalez-Alfonso, Francisco J. Plou, Julio Polaina, Julia Sanz-Aparicio, Manuel Ferrer, Víctor Guallar, David Talens-Perales
Format:	Article
Language:	English
Published:	Elsevier 2025-01-01
Subjects:	Feruloyl esterase Protein Engineering PluriZyme Xylan Xylanase
Online Access:	http://www.sciencedirect.com/science/article/pii/S2001037025003630