Directed evolution of aminoacyl-tRNA synthetases through in vivo hypermutation
Abstract Genetic code expansion (GCE) is a critical approach to the site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. Central to GCE is the development of orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs wherein engineered aaRSs recognize chosen ncAAs and charge t...
| Published in: | Nature Communications |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Online Access: | https://doi.org/10.1038/s41467-025-60120-w |