Simultaneous Identification of Functional Antigen-Specific CD8⁺ and CD4⁺ Cells after In Vitro Expansion Using Elongated Peptides

Elongated peptides (EPs), containing possibly one or multiple epitope/s, are increasingly used for the screening of antigen-specific CD8⁺ and CD4⁺ cell responses. Here, we present an in vitro protocol that allows the amplification of antigen-specific cel...

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Bibliographic Details

Published in:	Cells
Main Authors:	Juliane Schuhmacher, Leon Kleemann, Jennifer Rebecca Richardson, Elisa Rusch, Hans-Georg Rammensee, Cécile Gouttefangeas
Format:	Article
Language:	English
Published:	MDPI AG 2022-10-01
Subjects:	T cells immunomonitoring elongated peptides ELISpot intracellular cytokine staining
Online Access:	https://www.mdpi.com/2073-4409/11/21/3451