Protocol for flow cytometry-assisted single-nucleus RNA sequencing of human and mouse adipose tissue with sample multiplexing

Summary: Adipocyte size and fragility and commercial kit costs impose significant limitations on single-cell RNA sequencing of adipose tissue. Accordingly, we developed a workflow to isolate and sample-barcode nuclei from individual adipose tissue samples, integrating flow cytometry for quality cont...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Anton Gulko, Adam Essene, Dylan Matthew Belmont-Rausch, Molly Veregge, Deepti Pant, Danielle Tenen, Benedicte Schultz Kapel, Margo P. Emont, Tune H. Pers, Evan D. Rosen, Linus T. Tsai
Format:	Article
Language:	English
Published:	Elsevier 2024-03-01
Subjects:	Cell isolation Single Cell Cell separation/fractionation Flow Cytometry Genomics Sequencing
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724000583