In Vivo Cloning Strategy for Rhizobium Plasmids
We have developed a simple system to clone indigenous Rhizobium plasmids into E. coli. The strategy consists of three matings: the first is to insert Tn5 in the plasmid to be cloned, the second incorporates the integrative vector into the inserted Tn5 in the native Rhizobium plasmid, and the last ma...
| Published in: | BioTechniques |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-10-01
|
| Online Access: | https://www.future-science.com/doi/10.2144/02334st01 |