Infecting kidney organoids with a cDNA reporter clone of SARS-CoV-2

Summary: Induced pluripotent stem cell (iPSC)-derived kidney organoids can be used for disease modeling and drug testing. Here, we describe a protocol to prepare stocks of an infectious clone of SARS-CoV-2 expressing a stable mNeonGreen reporter (icSARS-CoV-2-mNG). We demonstrate the infection of ki...

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Bibliographic Details
Published in:STAR Protocols
Main Authors: Hyunjae Chung, Maxwell P. Bui-Marinos, Waleed Rahmani, Jennifer A. Corcoran, Justin Chun
Format: Article
Language:English
Published: Elsevier 2022-09-01
Subjects:
Cell-based assays
Stem cells
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200497X

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http://www.sciencedirect.com/science/article/pii/S266616672200497X

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