Protocol for real-time assessment of mitochondrial and glycolytic ATP production in patient-derived glioma stem-like cells

Summary: Glioma cells switch between energetic pathways to adapt and resist therapies. We present a protocol for measuring mitochondrial and glycolytic ATP rates in patient-derived glioma stem-like cells using a Seahorse XF ATP rate assay. We describe steps for growing 3D glioma stem-like cells, att...

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Bibliographic Details

Published in:	STAR Protocols
Main Authors:	Pratibha Sharma, Vinay K. Puduvalli
Format:	Article
Language:	English
Published:	Elsevier 2024-09-01
Subjects:	Cell Biology Cell culture Cell-based Assays Cancer Health Sciences Metabolism
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724003241